毛细管电泳

Abstract(#br)A carbon nanotube (CNT)-polydimethylsiloxane (PDMS) composite electrode was developed for the capillary electrophoretic determination of the bioactive constituents in Rhizoma Chuanxiong , a traditional Chinese medicine. The novel composite electrode was fabricated on the basis of the in situ polyaddition of curing agent-containing dimethyl siloxane oligomer in the presence of CNTs in the inner bore of a piece of fused silica capillary under heat.

In this paper, we present a micro-electro-mechanical-system based on a microcapillary electrophoresis chip device integrated with optical detection components, including a micro-focusing lens structure and buried optic fibers. This is a promising approach to enhance the optical signal of the laser-induced fluorescence system for biomedical detection applications. This study utilized microcapillary electrophoresis (micro-CE) chips with two specific polymer materials, polymethylmethacrylate (PMMA) and polydimethylsiloxane (PDMS).

Disposable Microchip-Based Electrochemical Detector Using Polydimethylsiloxane Channel and Indium Tin Oxide ElectrodeMicrochip;Capillary Electrophoresis;PDMS;ITO;Electrochemical Detection;We have developed a microsystem with a capillary electrophoresis (CE) and an electrochemical detector (ECD). The microfabricated CE-ECD systems are adequate for a disposable type and the characteristics are optimized for an application to the electrochemical detection. The system was realized with polydimethylsiloxane (PDMS)-glass chip and indium tin oxide electrode.

近年来，一种利用开放存取资源进行实验室研究的趋势已经开始。科学硬件的开源设计策略依赖于使用广泛可用的部件，特别是那些可以直接打印的部件，使用可与低成本微控制器连接的可加性制造技术和电子元件。开源软件消除了昂贵商业许可证的需要，提供了针对特定需求设计程序的机会。本综述对化学分离领域内的“开源运动”的影响进行了描述，主要是通过对近5年来该领域的研究进行全面的考察。涵盖的主题包括一般实验室设备、样品制备技术、基于分离的分析、检测策略、电子系统控制和数据处理软件。还讨论了在这些与分离有关的专题中继续存在的障碍和进一步采用开源办法的可能机会。

血流中的药物以自由和束缚两种形式存在，其中自由药物负责药理活性。由于蛋白质结合决定了药物在血液中的自由浓度，因此在药物发现和开发的早期阶段测定药物的蛋白质结合具有重要意义。此外，在个性化医疗和治疗药物监测中，最有利于测量药物的自由浓度。为此，需要灵敏、选择性好、快速的分析方法来测定药物的自由浓度及其与蛋白质结合的程度也随之增加。本综述旨在总结近年来用于测定游离药物浓度和血浆蛋白结合的分析方法的进展，重点介绍用于测定血浆蛋白结合的最重要方法。此外，还将介绍和讨论每种方法的概念及其固有的优缺点。