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On-chip magneto-immunoassay for Alzheimer's biomarker electrochemical detection by using quantum dots as labels

ty10086 提交于 周四, 08/26/2021 - 13:32
Abstract(#br)Electrochemical detection of cadmium-selenide/zinc-sulfide (CdSe@ZnS) quantum dots (QDs) as labeling carriers in an assay for apolipoprotein E (ApoE) detection has been evaluated. The immunocomplex was performed by using tosylactivated magnetic beads as preconcentration platform into a flexible hybrid polydimethylsiloxane (PDMS)-polycarbonate (PC) microfluidic chip with integrated screen printed electrodes (SPE). All the immunoassay was performed in chip and in flow mode. The sensitive electrochemical detection was obtained by square wave anodic stripping voltammetry.

药物未结合浓度和血浆蛋白结合测定的最新进展:分析方法

ty10086 提交于 周三, 08/25/2021 - 16:27
血流中的药物以自由和束缚两种形式存在,其中自由药物负责药理活性。由于蛋白质结合决定了药物在血液中的自由浓度,因此在药物发现和开发的早期阶段测定药物的蛋白质结合具有重要意义。此外,在个性化医疗和治疗药物监测中,最有利于测量药物的自由浓度。为此,需要灵敏、选择性好、快速的分析方法来测定药物的自由浓度及其与蛋白质结合的程度也随之增加。本综述旨在总结近年来用于测定游离药物浓度和血浆蛋白结合的分析方法的进展,重点介绍用于测定血浆蛋白结合的最重要方法。此外,还将介绍和讨论每种方法的概念及其固有的优缺点。