细胞粘附

Abstract(#br)BC (bacterial cellulose) exhibits quite distinctive properties than plant cellulose. The outstanding properties make BC a promising material for preparation of artificial blood vessel. By taking advantage of the high oxygen permeability of PDMS (polydimethylsiloxane) as a tubular template material, a series of BC tubes with a length of 100\u003cce:hsp sp=\"0.25\"/\u003emm, a thickness of 1\u003cce:hsp sp=\"0.25\"/\u003emm and an outer diameter of 4 or 6\u003cce:hsp sp=\"0.25\"/\u003emm were biosynthesized with the help of Gluconacetobacter xylinum .

Abstract(#br)Animal cells are generally cryopreserved in cryovials in a cell suspension state containing 5%–10% v/v dimethyl sulfoxide (DMSO) used as a cryoprotective agent. However, cryopreservation of cells in an attached state has not been intensively studied, and the effective freezing solution remains unknown. Here we determined the suitable DMSO concentration for the cryopreservation of human hepatoma HepG2 cells attached to glass and polydimethylsiloxane (PDMS) matrices coated with poly- l -lysine.

Abstract(#br)Sophisticated cell pattern provides unique cellular assay platform for studying cell to cell interaction, cellular differentiation and signaling, high-throughput cell response to chemicals. In this study, we demonstrated reactive oxygen species (ROS) mediated endothelial cell micropatterning on a polydimethylsiloxane (PDMS) substrate. The exposure of UV/O radiation led to the formation of ROS on the surface of PDMS, which could selectively prevent adhesion of endothelial cells.

Mammalian cells regulate adhesion by expressing and regulating a diverse array of cell adhesion molecules on their cell surfaces. Since different cell types express distinct sets of cell adhesion molecules, substrate-specific adhesion is cell type- and condition-dependent. Single-cell force spectroscopy is used to quantify the contribution of cell adhesion molecules to adhesion of cells to specific substrates at both the cell and single molecule level. However, the low throughput of single-cell adhesion experiments greatly limits the number of substrates that can be examined.

Background: Mechanosensing of cells, particularly the cellular response to substrates with different elastic properties, has been discovered in recent years, but almost exclusively in mammalian cells. Much less attention has been paid to mechanosensing in other cell systems, such as in eukaryotic human pathogens. Results: We report here on the influence of substrate stiffness on the adhesion of the human pathogen Acanthamoebae castellanii (A. castellanii) . By comparing the cell adhesion area of A.

表面性质对生物材料如何与环境相互作用起着关键作用。表面形貌在一些研究中也有报道有影响，尽管其作用仍未很好阐明。本研究通过化学腐蚀的中间表面制备了纳米粗糙化聚二甲基硅氧烷( PDMS )基底。此外，将含有10 ~ 30个 μm直径微柱的PDMS基底逐层功能化壳聚糖( CHI )和透明质酸( HA )多层膜，提交PC3肿瘤细胞黏附实验。利用原子力显微镜( AFM )对这些基底进行了表征，并估算了一些粗糙度参数。通过对形貌的统计描述，我们考察了这些表面参数对PC3细胞黏附的影响。AFM结果显示PDMS表面形貌发生了明显的改变，细胞粘附实验表明光滑的表面诱导PC3细胞粘附，尤其是Hurst指数高的表面。除了AFM分析外，还通过接触角和紫外可见测量监测了LbL功能化基底的表面修饰情况。功能化基底的润湿性改善和显著的Alcian Blue吸光度表明HA / CHI薄膜沉积成功。LbL功能化提高了PDMS基底的细胞捕获电位，其中较小直径的微柱有利于细胞的黏附机制。尽管仍需要做大量工作，但这些发现推动了对纳米分形粗糙度在细胞粘附中作用的基本认识的进展，并有助于开发在生物传感器等生物医学系统中应用的新型生物材料。