表面处理

Rapid, simple, and cost-effective treatments to achieve long-term hydrophilic PDMS surfaces

ty10086 提交于 周四, 08/26/2021 - 13:39
Abstract(#br)This paper describes rapid, simple, and cost-effective treatments for producing biocompatible and long-term hydrophilic polydimethylsiloxane (PDMS) surfaces identified in an experimental study investigating 39 treatments in all. The wetting of the surfaces was monitored during six months. Changes in surface morphology and chemical composition were also analyzed. Some of the treatments are presented here for the first time, while for earlier presented treatments the selection of investigated parameters was wider and the observation period for the surface wetting longer.

Micro-patterned cell-sheets fabricated with stamping-force-controlled micro-contact printing

ty10086 提交于 周四, 08/26/2021 - 13:32
Abstract(#br)Cell-sheet-engineering based regenerative medicine is successfully applied to clinical studies, though cell sheets contain uniformly distributed cells. For the further application to complex tissues/organs, cell sheets with a multi-cellular pattern were highly demanded. Micro-contact printing is a quite useful technique for patterning proteins contained in extracellular matrix (ECM). Because ECM is a kind of cellular adherent molecules, ECM-patterned cell culture surface is capable of aligning cells on the pattern of ECM.

Effect of wettability and surface roughness on the adhesion properties of collagen on PDMS films treated by capacitively coupled oxygen plasma

ty10086 提交于 周四, 08/26/2021 - 13:29
Abstract(#br)Direct chemical bonding of biomolecules to the surface of chemically inert polymers such as polydimethylsiloxane (PDMS) is not easily achieved. Therefore, pre-activation of such materials, followed by attachment of the biomolecule is necessary.(#br)This paper describes a procedure to functionalize a PDMS surface by oxygen-based plasma followed by the adhesion of collagen type I for the preparation of adhesive-free bilayer composite intended as skin substitute.

Transfer printing for preparing nanostructured PDMS film as flexible SERS active substrate

ty10086 提交于 周四, 08/26/2021 - 13:27
Abstract(#br)Flexible surface enhanced Raman scattering (SERS) substrate would offer a highly sensitive and rapid means for detecting ultra-low concentration of analytes on an irregular surface, enabling its arising applications in wearable and wipeable devices. In this study, we launched a transfer printing method to fabricate a flexible and low cost SERS substrate with an ordered nanostructure.

PDMS微流控装置内皮细胞播种的表面改性技术

ty10086 提交于 周三, 08/25/2021 - 16:37
微流控芯片实验室细胞培养技术通过提供减少样品和试剂的数量以及加强对细胞微环境的控制而得到了广泛的应用。聚二甲基硅氧烷( PDMS )因其制备工艺廉价易得、无毒、生物相容性好、透气性高、光学透明等优点,是微流控细胞培养装置常用的聚合物。然而PDMS固有的疏水性质使得细胞播种在PDMS表面时具有挑战性。PDMS表面的疏水性还允许对可能影响细胞行为和功能的小分子和生物分子进行非特异性吸收/吸附。PDMS表面的亲水改性对于成功的细胞播种是必不可少的。本综述整理了用于改善PDMS亲水性以利于内皮细胞在PDMS器件中播种的不同技术及其优缺点。

PDMS微流控装置内皮细胞播种的表面改性技术。

ty10086 提交于 周三, 08/25/2021 - 16:37
微流控芯片实验室细胞培养技术通过提供减少样品和试剂的数量以及加强对细胞微环境的控制而得到了广泛的应用。聚二甲基硅氧烷( PDMS )因其制备工艺廉价易得、无毒、生物相容性好、透气性高、光学透明等优点,是微流控细胞培养装置常用的聚合物。然而PDMS固有的疏水性质使得细胞播种在PDMS表面时具有挑战性。PDMS表面的疏水性还允许对可能影响细胞行为和功能的小分子和生物分子进行非特异性吸收/吸附。PDMS表面的亲水改性对于成功的细胞播种是必不可少的。本综述整理了用于改善PDMS亲水性以利于内皮细胞在PDMS器件中播种的不同技术及其优缺点。

软光掩模光刻和液滴铺展纳米印刷技术用于蓝宝石图形衬底的制作

ty10086 提交于 周三, 08/25/2021 - 16:02
本文提出了一种可用于接触型光刻的软光掩模制作的创新方法。它利用液滴铺展纳米压印技术,在玻璃基底上用含有图案表面微腔的软聚二甲基硅氧烷( PDMS )模具压印一滴碳黑光刻胶( PR )。通过对PDMS模具表面和玻璃基板表面进行适当的表面处理,可以将碳黑电阻反向填充到模具表面微腔中,从而得到柔软的PDMS光掩模。在4 ″蓝宝石衬底上进行了光刻图形实验研究,获得了特征尺寸约为1 ~ 2   μ m的六方排列PR微柱。成功制作了嵌有碳黑电阻的软PDMS光掩模。在4″蓝宝石晶片上也取得了优异的光刻图形化效果,显示了在发光二极管( LED )行业制造图形化蓝宝石衬底的潜力。将讨论所提出的软光掩模光刻技术的进一步发展及其他可能的应用。

纳米粒子在聚二甲基硅氧烷基微通道上吸附的表征

ty10086 提交于 周三, 08/25/2021 - 15:54
纳米粒子( NPs )被用于各种医药应用。外泌体( Exosomes )是一种生物衍生的纳米颗粒,是通过从体液中分离和浓缩而获得的生物标志物。基于聚二甲基硅氧烷( PDMS )的微通道具有高透气性和低细胞毒性等优点,适合于纳米颗粒的精细处理。然而,纳米颗粒的大比表面积可能导致非特异性吸附在器件基底上,从而造成样品的损失。因此,了解NP在微通道上的吸附情况对用于NP处理的微流控器件的运行至关重要。本文利用原子力显微镜对NP在PDMS基片和微通道上的吸附行为进行了表征,并将NP的吸附行为与NP的静电作用和分散介质性质联系起来。当聚苯乙烯NP分散体以恒定的流速引入PDMS基微通道时,随着NP和微通道zeta电位的降低(即随着pH的升高),吸附的纳米颗粒数量减少,表明微通道与纳米颗粒之间的静电作用增强了它们的斥力。在恒定流速下,将exosome分散体引入不同润湿性的PDMS微通道中,exosome的吸附以静电作用为主。所得结果将有助于PDMS基微流控装置对纳米颗粒的预富集、分离和传感。

纳米粒子在聚二甲基硅氧烷基微通道上吸附的表征。

ty10086 提交于 周三, 08/25/2021 - 15:54
纳米粒子( NPs )被用于各种医药应用。外泌体( Exosomes )是一种生物衍生的纳米颗粒,是通过从体液中分离和浓缩而获得的生物标志物。基于聚二甲基硅氧烷( PDMS )的微通道具有高透气性和低细胞毒性等优点,适合于纳米颗粒的精细处理。然而,纳米颗粒的大比表面积可能导致非特异性吸附在器件基底上,从而造成样品的损失。因此,了解NP在微通道上的吸附情况对用于NP处理的微流控器件的运行至关重要。本文利用原子力显微镜对NP在PDMS基片和微通道上的吸附行为进行了表征,并将NP的吸附行为与NP的静电作用和分散介质性质联系起来。当聚苯乙烯NP分散体以恒定的流速引入PDMS基微通道时,随着NP和微通道zeta电位的降低(即随着pH的升高),吸附的纳米颗粒数量减少,表明微通道与纳米颗粒之间的静电作用增强了它们的斥力。在恒定流速下,将exosome分散体引入不同润湿性的PDMS微通道中,exosome的吸附以静电作用为主。所得结果将有助于PDMS基微流控装置对纳米颗粒的预富集、分离和传感。