Development of an aptamer-based impedimetric bioassay using microfluidic system and magnetic separation for protein detection
Abstract(#br)An aptamer-based impedimetric bioassay using the microfluidic system and magnetic separation was developed for the sensitive and rapid detection of protein. The microfluidic impedance device was fabricated through integrating the gold interdigitated array microelectrode into a flow cell made of polydimethylsiloxane (PDMS). Aptamer modified magnetic beads were used to capture and separate the target protein, and concentrated into a suitable volume. Then the complexes were injected into the microfluidic flow cell for impedance measurement.