生物传感器

Abstract(#br)We will describe the use of an alternative polymer which is suitable for use in microfluidic applications which require the use of harsh chemicals or aggressive solvents. Currently microfluidics systems are mainly manufactured in polydimethylsiloxane (PDMS) due to its ease of use, and the ability to produce monolithic microfluidic valves directly into the same material as the microfluidic chip.

Abstract(#br)The present work deals with the development of a Love-wave bacteria-based sensor platform for the detection of heavy metals in liquid medium. The acoustic delay-line is inserted in an oscillation loop in order to record the resonance frequency in real-time.

Abstract(#br)The biological and medical application of biosensors is more and more important with the development of technology and society. Detection of cells and biological molecules utilizing biosensors based on the analysis of surface stress would facilitate inexpensive and high-throughput test and diagnosis. This paper presents a biocompatible surface stress-based polydimethylsiloxane (PDMS) micro membrane biosensor. Each biosensor chip consists of two available PDMS micro membranes, one acts as active membrane and the other as reference.

Abstract(#br)We present a microfluidic cell plate for endocrine disrupting chemicals (EDCs) detection, like estrogenic activity, in waterish solution. This platform technology consists of four independent micro flow units made of polydimethylsiloxane (PDMS) and glass, which enables a selective detection of up to four species of the EDCs per one-way chip containing the corresponding immobilized receptor. The concept of the detection is based on direct fluorescence analysis.

Abstract(#br)The hydrophobic characteristics of PDMS and non-specific protein adsorption are major drawbacks for its application in biosensing. Here we have combined surface oxidation by plasma and chemical binding of polyvinyl alcohol (PVA) to obtain long-term stability of hydrophilic PDMS surfaces. Mercaptopropyltrimethoxisilane and aminopropyltrimethoxisilane were used as adhesives between the plasma-oxidized PDMS surface and the PVA, immobilized at room temperature.

Abstract(#br)An aptamer-based impedimetric bioassay using the microfluidic system and magnetic separation was developed for the sensitive and rapid detection of protein. The microfluidic impedance device was fabricated through integrating the gold interdigitated array microelectrode into a flow cell made of polydimethylsiloxane (PDMS). Aptamer modified magnetic beads were used to capture and separate the target protein, and concentrated into a suitable volume. Then the complexes were injected into the microfluidic flow cell for impedance measurement.

Abstract(#br)We present a new diagnostic technique of fundamental monomeric biomaterials that do not rely on any enzyme or chemical reaction. Instead, it only uses radio frequency (RF) signal analysis. The detection and classification of basic biomaterials, such as glucose and albumin, were demonstrated. The device was designed to generate a strong resonance response with glucose solution and fabricated by simple photolithography with PDMS (Polydimethylsiloxane) well.

This work reports on diverse technologies implemented for fabricating microfluidic devices such as biomedical micro sensors, micro pumps, bioreactors and micro separators. UV depth lithography and soft lithography were applied in the fabrication processes using different materials, for example SU-8, polydimethylsiloxane (PDMS), silicon, glass and ceramics. Descriptions of the fabrication process of completed devices and their performance are provided. Experimental tests and results are presented where available.

Monodisperse and water‐soluble lanthanide (Ln\u003csup\u003e3+\u003c/sup\u003e)‐doped Y(Gd)VO\u003csub\u003e4\u003c/sub\u003e nanocrystals (NCs) have been successfully synthesized by a fast, facile, and environmentally friendly microwave‐assisted route with poly(acrylic acid) as the surfactant.

Alpha-fetoprotein (AFP) is a tumor-associated fetal protein that can be expressed in large amounts in adult tumor cells, serving as a useful clinical tumor-marker. Silicon nanowire (SiNW) biosensors have emerged as a powerful tool in detecting protein biomarkers, due to their ultrahigh sensitivity, real-time response and label-free detection. We fabricated a SiNW-based field-effect transistor (FET) according to \"top-down\" methodology. First, anti-AFP antibodies were immobilized onto the surface of the SiNW-FET.