生物膜

Abstract(#br)High-temperature-cured silicone rubber samples (silicone rubber (SIR) based on polydimethylsiloxane (PDMS)) and SIR samples containing three different antimicrobial agents, sodium benzoate (NaB), DCOIT (4,5 Dichloro-2-octyl-2H-isothiazolone-one) and p-aminobenzoic acid (PABA) were inoculated with fungal spore suspensions and incubated for 28 days at 29\u003cce:hsp sp=\"0.2\"/\u003e±\u003cce:hsp sp=\"0.2\"/\u003e1\u003cce:hsp sp=\"0.25\"/\u003e°C and ≥90% humidity, according to the ISO 846:1997(E) protocol.

Abstract(#br)Despite numerous preventive strategies on bacterial adhesion, pathogenic biofilm formation remained the major cause of medical device-related infections. Bacterial interference is a promising strategy that uses pre-established biofilms of benign bacteria to serve as live, protective coating against pathogen colonization. However, the application of this strategy to silicone urinary catheters was hampered by low adherence of benign bacteria onto silicone materials.

Abstract(#br)Active release antimicrobial coatings for medical devices have been developed to prevent and treat biofilm implant-related infections. To date, only a handful of coatings have been put into clinical use, with limited success. In this study, a novel antimicrobial compound was incorporated into a silicone (polydimethylsiloxane or PDMS) polymer to develop a novel active release coating that addressed several limitations of current device coatings.

Abstract(#br)Bacterial infection remains one of the most serious issues affecting the successful installation and retention of orthopedic implants. Many bacteria develop resistance to current antibiotics, which complicates or prevents traditional antibiotic-dependent eradication therapy. In this study, a hybrid coating of titanium dioxide and polydimethylsiloxane (PDMS) was synthesized to regulate the release of silver. The coatings were benefited from the antimicrobial activity of silver ion, the biocompatibility of titanium dioxide, and the flexibility of the polymer.

Abstract(#br)Antimycotic behavior of zinc oxide decorated with silver nanoparticles (ZnO-AgNPs) against Trichophyton mentagrophytes ( T. mentagrophytes ) was investigated on this work. Silver decoration size was modulated during Ag 2 O and Ag 0 synthesis in aqueous solution at pH\u003cce:hsp sp=\"0.25\"/\u003e11, 12 and 14, obtaining the smallest size at pH\u003cce:hsp sp=\"0.25\"/\u003e12 and the largest at pH\u003cce:hsp sp=\"0.25\"/\u003e14. In ex-situ tests, ZnO-AgNPs prevents successfully the growth of T.

Abstract(#br)Recent studies have proved the advantages of novel composite membranes in the extractive membrane bioreactor (EMBR) for recalcitrant organic wastewater treatment. However, more systematic research into membrane properties and mass transfer effects are required.

Abstract\n\t\t\t\t\n\t\t\t\t\tHigh-temperature-cured silicone rubber samples (silicone rubber (SIR) based on polydimethylsiloxane (PDMS)) and SIR samples containing three different antimicrobial agents, sodium benzoate (NaB), DCOIT (4,5 Dichloro-2-octyl-2H-isothiazolone-one) and p-aminobenzoic acid (PABA) were inoculated with fungal spore suspensions and incubated for 28 days at 29\n\t\t\t\t\t\t±\n\t\t\t\t\t\t1\n\t\t\t\t\t\t°C and ≥90% humidity, according to the ISO 846:1997(E) protocol.

囊性纤维化( Cystic fibrosis，CF )是一种常染色体隐性遗传病，可导致气道黏液增厚。这些条件有利于多菌性感染，如慢性肺部感染，其中铜绿假单胞菌( P . aeruginosa )是CF患者生命末期定植CF肺部的主要致病菌。这项体外研究使用铜绿假单胞菌生物膜模型，在部分囊性纤维化条件下，取样挥发性的胞外代谢产物。气体采样采用薄膜微萃取( TFME )和商用聚二甲基硅氧烷( PDMS )薄膜，负载薄膜分析采用气相色谱-四极杆质谱-热脱附( TD-GC-qMS )联用技术。为此，采用热重-大气压光离子化qMS ( TG- APPI-qMS )联用技术对两种市售薄膜进行了均一性和温度稳定性表征。采用本研究建立的方法对所选薄膜进行清洗。TD-GC-qMS法成功用于已知铜绿假单胞菌产生的挥发性代谢产物的标准。该方法在低纳米范围( 0.5 n M和1.5 n M )对中、低极性化合物的检测和定量均达到了极限。最后将所建立的方法应用于好氧和厌氧条件下铜绿假单胞菌DSM 50071生物膜产生的胞外挥发性代谢产物的研究。综上所述，两种条件下均能检测到11种代谢物。此外，本研究还表明，不同的氧气条件(有氧和无氧)会产生不同的胞外挥发性代谢产物。特定代谢物，如1 -十一烯(有氧)和2 -十一酮(无氧)，可以被鉴定。该结果很有前途，因为生物膜模型可能适用于临床条件下铜绿假单胞菌的鉴定。

研究了一种由聚二甲基硅氧烷( PDMS )与粉末金属银、锌混合而成的导管涂层。该涂层易于应用于现有的有机硅器件，并能有效抑制生物膜的形成。涂层制备完成后，通过扫描电子显微镜( SEM )和能量色散X射线( EDX )分析其表面材料特性，在标准硅导管表面涂复薄膜，考察涂层的抗菌性能。人们发现一种独特的锌银涂层配方在6d的时间内抑制大肠杆菌的生物膜和浮游生长，似乎与重金属离子的释放、电场的产生、过氧化氢的生成或这些效应的组合有关。在去离子水和尿液中，锌和银箔开路电位分别为1.2   V和0.97   V。抑制区的实验结果表明，使用混合金属时银离子扩散的可能性，因为细菌的形成被抑制在样品边缘以外。对所有样品进行了13天的过氧化氢测定。含锌样品被发现能产生过氧化氢，一种已知的抗菌剂。样品的润湿性测试表明，活性物质的引入导致亲水性增加，可能影响细菌的粘附。

商业和实验抗菌导尿管均考察其在人工合成尿液中防止大肠杆菌浮游生长和生物膜形成的功效。聚二甲基硅氧烷( PDMS )粘结剂中Ag、Ag / Ag2O或Zn / Ag2O分散较薄( \u003c 500 µ m )的实验抗菌导管均表现出明显的抗菌活性，优于传统商业抗菌导管。所有实验导管使用集落形成单位( CFU )测定法防止细菌浮游生物生长，在6天的试验期间没有出现生物膜形成。另一方面，性能最好的商业导管在浮游生物生长试验中仅表现出3天的功效，并在CFU测量中形成多个细菌菌落。Zn / Ag2O / PDMS实验导管是唯一能产生过氧化氢的导管，这是一种已知的抑制生物膜形成的活性氧，而Ag2O / PDMS和Ag / Ag2O / PDMS实验导管没有检测到过氧化氢的产生，这表明杀菌作用很可能是由于PDMS涂层中银离子的释放。