Microfluidic system for cell fusion
Abstract(#br)We present a microfluidic chip for cell handling applications fabricated in polydimethylsiloxane (PDMS). The chip was designed to accumulate cells inside a reaction chamber where they can be manipulated under attendance of a liquid reagent (PEG–polyethylene glycol) for cell fusion. To enable the cell handling a fluid management system was developed which includes pneumatically actuated microfluidic valves integrated into the chip. Various valve geometries were simulated in ANSYS and characterized in a series of tests to identify a feasible valve concept.