气动驱动

Abstract(#br)We present a microfluidic chip for cell handling applications fabricated in polydimethylsiloxane (PDMS). The chip was designed to accumulate cells inside a reaction chamber where they can be manipulated under attendance of a liquid reagent (PEG–polyethylene glycol) for cell fusion. To enable the cell handling a fluid management system was developed which includes pneumatically actuated microfluidic valves integrated into the chip. Various valve geometries were simulated in ANSYS and characterized in a series of tests to identify a feasible valve concept.

In this paper we present various pneumatically actuated microfluidic valves to enable user-defined fluid management within a microfluidic chip. To identify a feasible valve design, certain valve concepts are simulated in ANSYS to investigate the pressure dependent opening and closing characteristics of each design. The results are verified in a series of tests. Both the microfluidic layer and the pneumatic layer are realized by means of soft-lithographic techniques. In this way, a network of channels is fabricated in photoresist as a molding master.

本文提出了一种自容式微光学系统，通过气压调节微透镜在器件中的位置，实现放大控制。与传统的由液体或聚二甲基硅氧烷( PDMS )通过外部驱动改变其形状的动态微透镜不同，该系统将固定曲率的玻璃微透镜、可充气的PDMS层和外部气动气压供给作为驱动器。该装置具有充气稳定、结构牢固、重量轻等优点，利用玻璃微透镜结构实现了比以往报道更大的位移。该微透镜由120 µ m厚的平板薄玻片制成，通过100 µ m厚PDMS膜的偏转来调节系统放大倍数，从而改变微透镜到微流控通道的距离。系统放大倍数与施加在装置上的气压成正比，在2.5mbar气压下，实现2.2X的放大倍数。这种光学系统对于各种短工作距离观测任务与高分辨能力显微镜结合是理想的，特别有利于各种基于芯片的分析。