Enzyme-coated microelectrodes to monitor lactate production in a nanoliter microfluidic cell culture device
Abstract(#br)Monitoring the degree of anaerobic respiration of cells in high density microscale culture systems is an enabling key technology and essential for cell-based biosensors. We have fabricated and incorporated miniature amperometric lactate sensing electrodes with working areas from 3 to 5\u003cce:hsp sp=\"0.25\"/\u003e×\u003cce:hsp sp=\"0.25\"/\u003e10 −2 \u003cce:hsp sp=\"0.25\"/\u003emm 2 into a microfluidic-based microscale cell culture system to measure the lactate production rate of fibroblasts in nanoliter volumes.