肝细胞

Abstract(#br)After isolation from a living organism, primary hepatocytes markedly change their morphology and lose specific functions. This study aimed to utilize a simple solution-phase surface modification method to fabricate a galactosylated polydimethylsiloxane (GAL-PDMS) substratum. Using both Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) analysis, we demonstrated that the surface of PDMS was galactosylated.

Abstract(#br)Since oxygen is one of the critical limiting factors for maintaining cell viability and function, a great deal of effort is being focused on improving the oxygen supply to three-dimensional (3D) cellular constructs. Here, we report a technique to construct spheroids utilizing 3D culture chips with a rapid and simple method for the replication of the surface structures of a polydimethylsiloxane (PDMS) mold.