趋化性

Abstract(#br)We describe here a novel real-time cell tracking system which can measure cell migration routes under cell culture condition. This system includes a mini incubator which controls temperature and CO 2 gas flow and a PDMS (polydimethylsiloxane) chip for chemotaxis measurement. The main differences from previous ones are real-timely long-term (⩾24\u003cce:hsp sp=\"0.25\"/\u003eh) tracking for single cell quantitatively, simple and inexpensive constitution of optical parts for illumination and imaging, and compatible to commercial well plate.

Abstract(#br)We developed and examined a toxicity sensing microchip, where 200-300 cells of flagellated microbes ( Euglena gracilis ) were placed and their movements were monitored with a video camera. The microchip had two microchannels for test sample and reference flow, and the cells were confined completely in a separated closed-space (micro-aquarium). The two microchannels run aside of the micro-aquarium, and molecules of test sample permeated into the micro-aquarium from the microchannels via diffusion through porous polydimethylsiloxane (PDMS) wall.