Toxicity Sensing by Using Chemotactic Reaction of Microbial Cells Confined in Microfluidic Chip
Abstract(#br)We developed and examined a toxicity sensing microchip, where 200-300 cells of flagellated microbes ( Euglena gracilis ) were placed and their movements were monitored with a video camera. The microchip had two microchannels for test sample and reference flow, and the cells were confined completely in a separated closed-space (micro-aquarium). The two microchannels run aside of the micro-aquarium, and molecules of test sample permeated into the micro-aquarium from the microchannels via diffusion through porous polydimethylsiloxane (PDMS) wall.