In vitro and in vivo evaluation of the antibacterial properties of a nisin-grafted hydrated mucin multilayer film
Abstract(#br)Microbial infection is one of the most serious problems in the field of medical devices, particularly in implants. Herein, we have designed and constructed a (mucin/poly(ethyleneimine)) n ((mucin/PEI) n ) multilayer film using a layer-by-layer self-assembly method and the grafting of antimicrobial peptides to enhance the bactericidal efficacy. Water contact angle measurements and atomic force microscopy images revealed that the hydrated multilayer film created a highly hydrophilic surface with a low roughness.