Fabrication and characterization of a flow-through PCR device with integrated chromium resistive heaters
Abstract\n\t\t\t\t\n\t\t\t\t\tA flow-through PCR device with integrated chromium resistive heaters was developed. The PCR device was composed of a polydimethylsiloxane flow channel chip fabricated using soft lithography and a glass heating chip produced by standard photolithography. PDMS bonding layers were used to assemble the device instead of oxygen plasma treatment. The formation of air bubbles within the device was suppressed by treating the flow channel with 20% (v/v) Tween 20.