高效液相色谱(HPLC)

Abstract(#br)In this study, a porous polypropylene frit was coated with polydimethylsiloxane (PDMS) as extraction medium, based on the home-made PDMS-frit, a rapid, simple and sensitive sorptive extraction method was established for analysis of potential biomarkers of lung cancer (hexanal and heptanal) in human serum samples. In the method, derivatization and extraction occurred simultaneously on the PDMS-frit, then the loaded frit was ultrasonically desorbed in acetonitrile. Polymerization, derivatization–extraction and desorption conditions were optimized.

多环芳烃( PAHs )代表一类有机物。鱼油已被确定为食品中持久性有机污染物水平的最重要贡献者之一。事实上，由于鱼类的代谢能力较低，鱼油中可能含有较高水平的亲脂性污染物，如PAHs。鱼油中PAHs的测定由于脂肪基质的存在而比较复杂，对提取效率和分析数据质量都有不利影响。利用织物相吸附萃取( FPSE )从营养补充剂中成功提取目标化合物，为洗脱PAHs，将制备好的溶胶-凝胶苯基/聚二甲基硅氧烷( PDMS )涂层织物相吸附萃取膜用乙腈反萃取，洗脱后的样品用荧光检测的高效液相色谱( HPLC )分析。PAHs在C18 125×4 mm，5μm色谱柱上洗脱，烘箱温度30℃，乙腈和水梯度洗脱。分别在270和390 nm波长处测定芘和芘，290和430 nm波长处测定苯并( a )芘，方法的线性范围(芘和芘2.5 ~ 75 ng / m L，苯并( a )芘2.5 ~ 100 ng / m L )、精密度和真实性(日内和日间)、回收率( 63.2 % ~ 102.3 % )均得到验证。本工作首次提出了一种新的FPSE采样/样品清理程序，该程序最近在生物、环境、化妆品和食品基质的分析中显示了其优势。