细胞形态

Abstract(#br)There is a need for an artificial salivary gland as a long-term remedy for patients suffering from salivary hypofunction, a leading cause of chronic xerostomia (dry mouth). Current salivary gland tissue engineering approaches are limited in that they either lack sufficient physical cues and surface area needed to facilitate epithelial cell differentiation, or they fail to provide a mechanism for assembling an interconnected branched network of cells.

In vitro Cytotoxicity Evaluation of Polydimethylsiloxane as a Biosensor Coating Materialcytotoxicity;biocompatibility;polydimethylsiloxane;MTT;cell morphology;PDMS was selected for a coating material of implantable biosensors and the cytotoxicity of extracts released from a polymer was evaluated using ISO 10993-5, Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. Organo-tin was used as a positive control and a medium without serum was used as a negative control.

细胞的微环境已被证明对细胞行为产生影响。特别是基质-细胞相互作用强烈影响细胞形态和功能。本研究旨在分析不同培养基质材料对肺上皮腺癌( A549 )细胞表型和功能特性的影响。将A549细胞接种于两种不同的生物相容性、市售的基质上：作为细胞培养板对照的聚酯玻片( Thermanox ™ Coverskis )和聚二甲基硅氧烷膜( PDMS，Elastosil® Film )。两种底物均影响细胞形态和肌动蛋白细胞骨架组织。进一步，在聚酯基底上培养的A549细胞中，Yes相关蛋白( YAP )及其转录共激活因子PDZ结合基序( TAZ )被转位到细胞核中，但在PDMS基底上的细胞中仍多保留胞浆。通过SEM分析，我们观察到生长在Elastosil®膜上的细胞保持肺泡Ⅱ型细胞形态。表面活性剂-C免疫荧光染色显示，表面活性剂-C在Elastosil®膜上培养的细胞中高表达，而在Thermanox ™盖玻片上培养的细胞中不表达。生长在Elastosil®膜上的A549细胞的形态和功能显示肺泡上皮Ⅱ型表型的保留，而生长在常规塑料基质上的A549细胞获得肺泡Ⅰ型表型。