蛋白质

Abstract(#br)Coupling infrared (IR) spectroscopy to microfluidic devices provides a powerful tool for characterizing complex chemical and biochemical reactions. Examples of microfluidic devices coupled with infrared spectroscopy have been limited, however, largely due to the difficulties associated with fabricating systems in common infrared transparent materials like CaF 2 . Recent reports have shown that polydimethylsiloxane (PDMS) can be used as an IR transparent substrate when fabricated with thin layers.

A protein digestion system using immobilized enzymes for protein identification and glycochain analyses has been developed, and a vibration reaction unit for micro-scale sample convection on an enzyme-immobilized solid surface was constructed. BSA as a model substrate was digested by this unit, and was successfully identified by mass spectrometry (MS) analyses. Compared to the conventional liquid-phase digestion, the reaction unit increased the number of matched peptides from 9 to 26, protein score from 455 to 1247, and sequence coverage from 21% to 48%.

A protein digestion system using immobilized enzymes for protein identification and glycochain analyses has been developed, and a vibration reaction unit for micro-scale sample convection on an enzyme-immobilized solid surface was constructed. BSA as a model substrate was digested by this unit, and was successfully identified by mass spectrometry (MS) analyses. Compared to the conventional liquid-phase digestion, the reaction unit increased the number of matched peptides from 9 to 26, protein score from 455 to 1247, and sequence coverage from 21% to 48%.

以心肌肌钙蛋白I ( cardiac troponin I，Troponin I )为例，提出了一种电化学阻抗谱( EIS )微流控芯片作为生物传感器用于蛋白质的无标记检测。这里介绍的微流控阻抗生物传感器芯片由作为基板的显微镜载玻片、溅射电极和聚二甲基硅氧烷( PDMS )微通道组成。除了分析物特异性结合相应抗体和减少非特异性蛋白吸附以防止假阳性信号外，还考虑了可能的电荷通过传感层传递，制定了电极功能化协议。金电极上自组装单层( SAMs )所测试的试剂包括巯基化碳氢化合物和巯基化寡核苷酸，其中基于后者的SAMs表现出更好的性能。用碳二亚胺化学将相应抗体共价偶联在SAM上。取样和测量只用了几分钟。人血清白蛋白( HSA )样品1000ng / mL的应用导致阻抗变化微乎其微，而肌钙蛋白I样品1ng / mL的应用导致奈奎斯特图发生明显变化。该结果有望应用新开发的微流控阻抗生物传感器芯片特异性检测临床相关浓度的生物标志物，如心脏标志物。

以心肌肌钙蛋白I ( cardiac troponin I，Troponin I )为例，提出了一种电化学阻抗谱( EIS )微流控芯片作为生物传感器用于蛋白质的无标记检测。这里介绍的微流控阻抗生物传感器芯片由作为基板的显微镜载玻片、溅射电极和聚二甲基硅氧烷( PDMS )微通道组成。除了分析物特异性结合相应抗体和减少非特异性蛋白吸附以防止假阳性信号外，还考虑了可能的电荷通过传感层传递，制定了电极功能化协议。金电极上自组装单层( SAMs )所测试的试剂包括巯基化碳氢化合物和巯基化寡核苷酸，其中基于后者的SAMs表现出更好的性能。用碳二亚胺化学将相应抗体共价偶联在SAM上。取样和测量只用了几分钟。人血清白蛋白( HSA )样品1000ng / mL的应用导致阻抗变化微乎其微，而肌钙蛋白I样品1ng / mL的应用导致奈奎斯特图发生明显变化。该结果有望应用新开发的微流控阻抗生物传感器芯片特异性检测临床相关浓度的生物标志物，如心脏标志物。