免疫测定

Abstract(#br)An ideal microfluidic-based biosensor flow cell should have not only a “soft” interface for high strength sealing with biosensing chips, but also “hard” macro-to-micro interface for tubing connection. Since these properties are exclusive of each other, no one material can provide the advantages of both.

Abstract(#br)This article reports on the fabrication of a disposable bio-nano-chip (BNC), a microfluidic device composed of polydimethylsiloxane (PDMS) and thiolene-based optical epoxy which is both cost-effective and suitable for high performance immunoassays.

Abstract(#br)Here we present a capillary-driven microfluidic chip for “one-step” immunoassays. The chip allows for easy modification of several assay parameters such as the flow rates of sample, the volumes of samples for tests, and the type of reagents and receptors for detecting analytes. We therefore term such a chip a multiparametric chip and illustrate this concept with the integration and release of anti-C-reactive protein (CRP) detection antibodies (dAbs) together with splitting flow of samples containing CRP across lines of anti-CRP capture antibodies (cAbs).