器官培养

Abstract(#br)We compared the migration of liver and kidney explants, harvested from chicken embryos, cultivated inside trapezoidal microchannels and onto flat samples made of polydimethylsiloxane (PDMS). In all cases, liver migration was faster and greater than kidney migration. The microchannels enhanced 3D migration when compared to the flat samples, which made only 2D migration possible. However, migration velocity was found to be higher in 2D than in 3D migration.

AbstractIn our previous study, we produced a microfluidic device (MFD) which successfully maintained spermatogenesis for over 6 months in mouse testis tissues loaded in the device. In the present study, we developed a new MFD, a monolayer device (ML-D) with a barrier structure consisting of pillars and slits, which is simpler in design and easier to make. This ML-D was also effective for inducing mouse spermatogenesis and maintained it for a longer period than the conventional culture method.