细胞力学

摘要( # br ) PDMS作为一种广泛应用于细胞力学研究的弹性体，在细胞培养制备过程中受到多种表面处理。特别是考虑到其粘弹性性质，上述处理对PDMS力学行为的影响，尤其是在相关长度尺度为100 μ m时，受到的关注有限。尽管由于PDMS力学性能的微小变化，在定量细胞牵引力方面报告了重大误差。因此，本文通过拉伸和应力松弛实验，研究了等离子体氧化、灭菌和孵育对PDMS弹性模量、松弛模量和泊松比的影响，并通过线性黏弹性方程解释了后者的结果。观察到，虽然通过这一循环的表面处理测量了与未处理PDMS性能的显著偏差，但未经处理的PDMS在细胞培养基中孵育后性能几乎恢复。例如，处理后的PDMS的弹性模量比未处理的PDMS小6 %，对应的松弛模量和时均泊松比偏差分别\u003c 3 %和\u003c 1 %。在细胞培养基中培养过程结束时，泊松比随时间的变化率也被发现降低。因此，未处理PDMS的黏弹性性能可以在本工作提供的误差范围内安全使用。

Details are presented for the formulation, fabrication, and mechanical characterization of mesoscopic freestanding polydimethylsiloxane (PDMS) elastomer membranes, 10.0 microm thick and 5.0 mm in diameter, used to probe the rheology of a living epithelial sheet. In what is described as a composite diaphragm inflation (CDI) experiment, freestanding PDMS membranes are utilized as substrates for the culture of a sheet of epithelial cells.

Abstract\n\t\t\t\t\n\t\t\t\t\tDetails are presented for the formulation, fabrication, and mechanical characterization of mesoscopic freestanding polydimethylsiloxane (PDMS) elastomer membranes, 10.0 μm thick and 5.0 mm in diameter, used to probe the rheology of a living epithelial sheet. In what is described as a composite diaphragm inflation (CDI) experiment, freestanding PDMS membranes are utilized as substrates for the culture of a sheet of epithelial cells.