药物

\u003cabstract language=\"eng\"\u003eA novel solventless sample preparation, stir-bar sorptive extraction (SBSE), for extraction, and sample enrichment of organic compounds from biological fluids, is described in this manuscript from principle to applications. The SBSE is based on sorptive extraction, whereby the compounds are extracted into a polymer coating, polydimethylsiloxane (PDMS), on a magnetic stirring rod. The extraction is controlled by the partitioning coefficient of drugs between the PDMS and sample matrix, and upon the sample-extraction medium phase ratio.

摘要\n体上芯片和器官上芯片系统使用聚二甲基硅氧烷( PDMS )，因为材料相对适合制造这些器件中使用的微流控通道和腔室。然而，疏水性分子，特别是治疗性化合物，往往会吸附到PDMS上，这可能会扭曲馈入用于将临床前数据转化为临床结局预测的药动学/药效学模型的剂量-反应曲线。有机硅烷表面改性是目前正在探索的改性PDMS的方法之一，但有机硅烷对药物吸附等温线的影响还没有很好的表征。采用反相液-固相色谱仪对药物对乙酰氨基酚、双氯芬酸和维拉帕米在PDMS和有机硅烷修饰的PDMS表面的吸附参数进行了表征，并将修饰与药物吸附的变化进行了关联。利用我们的方法确定有机硅烷修饰显著改变了试验药物的吸附能。