浓度梯度

Abstract(#br)A simple but robust droplet-based microfluidic system was developed for dose–response enzyme inhibition assay by combining concentration gradient generation method with electrochemical detection method. A slotted-vials array and a tapered tip capillary were used for reagents introduction and concentration gradient generation, and a polydimethylsiloxane (PDMS) microfluidic chip integrated with microelectrodes was used for droplet generation and electrochemical detection. Effects of oil flow rate and surfactant on electrochemical sensing were investigated.

在许多生化实验中，必须控制特定位置的浓度梯度。本文提出了一种基于聚二甲基硅氧烷( PDMS )气体渗透性的可控位置，由声振气泡俘获在气泡通道内驱动的可调谐浓度梯度发生器。梯度发生器由玻璃基板、PDMS芯片和压电换能器组成。当被困气泡被声波激活后，气液界面附近的溶液发生混合。气泡的体积和气液界面的位置通过PDMS壁面的渗透性来调节。通过改变主通道中能够使流体混合的气泡的数量和位置，以及外加电压的幅值，可以实现浓度梯度的可调谐。这种新型器件易于制备、响应快、生物相容性好，具有很大的应用前景。特别适用于对时间可控性要求较高的生物研究。