心脏组织工程

Abstract(#br)Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) have been widely proposed as in vitro models of myocardial physiology and disease. A significant obstacle, however, is their immature phenotype. We hypothesised that Ca 2+ cycling of iPSC-CM is influenced by culture conditions and can be manipulated to obtain a more mature cellular behaviour. To test this hypothesis we seeded iPSC-CM onto fibronectin coated microgrooved polydimethylsiloxane (PDMS) scaffolds fabricated using photolithography, or onto unstructured PDMS membrane.

地形和机械特征是细胞发育的两个关键因素。在此，我们引入了一种易于制作的Mat-on-Gel结构来研究纳米几何和刚度对体外心肌细胞功能发育的共同影响。该结构由电纺纤维和聚二甲基硅氧烷( PDMS )凝胶组成，具有纳米纤维结构和可调的刚度。利用CM细胞系HL - 1细胞，对19 ~ 52  kPa三种不同刚度进行了比较研究。物理表征表明，与PDMS凝胶相比，Mat-on- Gel结构具有更好的亲水性，杨氏模量降低。从而使细胞黏附更好，细胞代谢活性增强。与单独在电纺纤维或PDMS凝胶上培养的CMs相比，Mat-on- Gel结构上的CMs也表现出更同步的电生理活性。尤其是较低的凝胶刚度导致更快的钙波。本研究综合论证了这种Mat-on-Gel结构的可行性及其作为体外药物研究平台的潜在用途。\n本文为心肌细胞提供了一种混合的Mat-on-Gel结构。它由聚二甲基硅氧烷( PDMS )凝胶顶部的一层电纺纤维组成，提供纳米几何尺寸和可调刚度。发现较软的基底诱导较快的钙波，而取向纤维则加速规则的钙瞬态发展。