灭菌

A novel comprehensive approach for human vascular allografts cryopreservation and radiation sterilization for the tissue engineering industry

ty10086 提交于 周四, 08/26/2021 - 13:06
Aim: to verify new techniques for human cadaveric vascular allografts cryopreservation, thawing and sterilization for the tissue engineering purposes. We use polydimethylsiloxane (PDMS) as a well-known, promising coolant. This allowed us to completely omit any cryoprotective or vitrifying solutions. Using of PDMS also makes possible an applying these allografts directly after freezing and decellularization and also it will also provide an opportunity to develop secure protocols of tissue— engineered vascular conduits cryopreservation. Matherial and methods.

生物医学应用聚二甲基硅氧烷( PDMS )微通道的制备与分析

ty10086 提交于 周三, 08/25/2021 - 16:26
在本研究工作中,聚二甲基硅氧烷( PDMS )已被用于制备用于生物医学应用的微通道。在基于物联网( IoT )的控制环境下,为了避免组织损伤,作者模拟并制备了生物可耐受、生物相容性和生物工程化的PDMS基静脉曲张微通道。利用MATLAB ( The Math-Works,Natick,MA,USA )和ANSYS ( ANSYS,Lahore大学,巴基斯坦)模拟了5个弯曲上升曲线微通道( 5CACMC )和5个弯曲下降曲线微通道( 5CDCMC ),并结合实际环境进行了实验验证。各通道总长1.6 cm。两通道直径均为400 µ m。在上升通道中,第一至第五曲线周期半径分别为2.5 mm、5 mm、7.5 mm、10 mm和2.5 mm。在下降通道中,第一和第二曲线周期半径分别为12.5 mm和10 mm。第三至第五周期半径分别为7.5 mm、5 mm和2.5 mm。对于5CACMC,在雷诺数为185时,模糊模拟的流量、速度和压降分别为19.7 µ Ls - 1、0.105 mm / s和1.18 Pa,ANSYS模拟的流量、速度和压降分别为19.3 µ Ls - 1、0.1543 mm / s和1.6 Pa,实验的流量、速度和压降分别为18.23 µ Ls - 1、0.1332 mm / s和1.5 Pa。