Protein adsorption capability on polyurethane and modified-polyurethane membrane for periodontal guided tissue regeneration applications

ty10086 提交于 周四, 08/26/2021 - 13:26
文章英文标题
Protein adsorption capability on polyurethane and modified-polyurethane membrane for periodontal guided tissue regeneration applications
正文
Abstract(#br)Periodontal disease if left untreated can result in creation of defects within the alveolar ridge. Barrier membranes are frequently used with or without bone replacement graft materials for achieving periodontal guided tissue regeneration (GTR). Surface properties of barrier membranes play a vital role in their functionality and clinical success. In this study polyetherurethane (PEU) membranes were synthesized by using 4,4′-methylene-diphenyl diisocyanate (MDI), polytetramethylene oxide (PTMO) and 1,4-butane diol (BDO) as a chain extender via solution polymerization. Hydroxyl terminated polydimethylsiloxane (PDMS) due to having inherent surface orientation towards air was used for surface modification of PEU on one side of the membranes. This resulting membranes had one surface being PEU and the other being PDMS coated PEU. The prepared membranes were treated with solutions of bovine serum albumin (BSA) in de-ionized water at 37\u003cce:hsp sp=\"0.25\"/\u003e°C at a pH of 7.2. The surface protein adsorptive potential of PEU membranes was observed using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Raman spectroscopy and Confocal Raman spectroscopy. The contact angle measurement, tensile strength and modulus of prepared membranes were also evaluated. PEU membrane (89.86\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1.62°) exhibited less hydrophobic behavior than PEU-PDMS (105.87\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e3.16°). The ultimate tensile strength and elastic modulus of PEU (27\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1\u003cce:hsp sp=\"0.25\"/\u003eMPa and 14\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e2\u003cce:hsp sp=\"0.25\"/\u003eMPa) and PEU-PDMS (8\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1\u003cce:hsp sp=\"0.25\"/\u003eMPa and 26\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1\u003cce:hsp sp=\"0.25\"/\u003eMPa) membranes was in required range. The spectral analysis revealed adsorption of BSA proteins on the surface of non PDMS coated PEU surface. The PDMS modified PEU membranes demonstrated a lack of BSA adsorption. The non PDMS coated side of the membrane which adsorbs proteins could potentially be used facing towards the defect attracting growth factors for periodontal tissue regeneration. Whereas, the PDMS coated side could serve as an occlusive barrier for preventing gingival epithelial cells from proliferating and migrating into the defect space by facing the soft tissue flaps. This study demonstrates the potential of a dual natured PEU barrier membrane for use in periodontal tissue engineering applications and further investigations are required.
文章内容(英文)
Abstract(#br)Periodontal disease if left untreated can result in creation of defects within the alveolar ridge. Barrier membranes are frequently used with or without bone replacement graft materials for achieving periodontal guided tissue regeneration (GTR). Surface properties of barrier membranes play a vital role in their functionality and clinical success. In this study polyetherurethane (PEU) membranes were synthesized by using 4,4′-methylene-diphenyl diisocyanate (MDI), polytetramethylene oxide (PTMO) and 1,4-butane diol (BDO) as a chain extender via solution polymerization. Hydroxyl terminated polydimethylsiloxane (PDMS) due to having inherent surface orientation towards air was used for surface modification of PEU on one side of the membranes. This resulting membranes had one surface being PEU and the other being PDMS coated PEU. The prepared membranes were treated with solutions of bovine serum albumin (BSA) in de-ionized water at 37\u003cce:hsp sp=\"0.25\"/\u003e°C at a pH of 7.2. The surface protein adsorptive potential of PEU membranes was observed using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Raman spectroscopy and Confocal Raman spectroscopy. The contact angle measurement, tensile strength and modulus of prepared membranes were also evaluated. PEU membrane (89.86\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1.62°) exhibited less hydrophobic behavior than PEU-PDMS (105.87\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e3.16°). The ultimate tensile strength and elastic modulus of PEU (27\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1\u003cce:hsp sp=\"0.25\"/\u003eMPa and 14\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e2\u003cce:hsp sp=\"0.25\"/\u003eMPa) and PEU-PDMS (8\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1\u003cce:hsp sp=\"0.25\"/\u003eMPa and 26\u003cce:hsp sp=\"0.25\"/\u003e±\u003cce:hsp sp=\"0.25\"/\u003e1\u003cce:hsp sp=\"0.25\"/\u003eMPa) membranes was in required range. The spectral analysis revealed adsorption of BSA proteins on the surface of non PDMS coated PEU surface. The PDMS modified PEU membranes demonstrated a lack of BSA adsorption. The non PDMS coated side of the membrane which adsorbs proteins could potentially be used facing towards the defect attracting growth factors for periodontal tissue regeneration. Whereas, the PDMS coated side could serve as an occlusive barrier for preventing gingival epithelial cells from proliferating and migrating into the defect space by facing the soft tissue flaps. This study demonstrates the potential of a dual natured PEU barrier membrane for use in periodontal tissue engineering applications and further investigations are required.
来源出处
Journal|[J]Materials Science and Engineering: CVolume 68, 2016. PP 267-275
DOI
https://doi.org/10.1016/j.msec.2016.05.026

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备注说明:

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