开发具有圆柱体微柱体的聚二甲基硅氧烷基仿生支架用于视网膜色素上皮细胞培养

ty10086 提交于 周三, 08/25/2021 - 16:43
文章英文标题
Development of polydimethylsiloxane-based biomimetic scaffolds with cylinder micropillars for retinal pigment epithelial cell cultivation
正文
背景:\n年龄相关性黄斑变性( AMD )是导致视力丧失的主要原因之一。一旦视网膜色素上皮( RPE )层被破坏,视力和认可度差一般是不可逆的。在再生医学中具有巨大潜力的细胞疗法可能为AMD等几种不治之症提供替代治疗。本研究开发了一种具有圆柱形微柱体的新型聚二甲基硅氧烷( PDMS )仿生支架,用于诱导多能干细胞源性RPEs ( iPSC-RPEs )的培养。RPEs在PDMS仿生支架上培养,验证细胞基因表达。\n方法:\n采用旋涂法和光刻法制备了仿生PDMS支架。它与生物分子进一步修饰在表面,以提高细胞亲和力和稳定性。将iPSC-RPEs接种于支架上,进行特征基因表达分析。\n结果:\n利用傅里叶变换红外光谱对PDMS仿生支架进行分析,证明其化学组成,iPSC-RPEs在支架上呈现融合细胞单层,并保持RPE特异性基因表达,证明PDMS仿生支架支持iPSC-RPEs生长。\n结论:\n PDMS界面允许iPSC-RPEs有规律生长,圆柱微柱的设计进一步提供了生物支架高运动阻力可能提高iPSC-RPEs移植后的移植稳定性。综合来看,这种创新性的PDMS基仿生支架可能作为体外iPSC-RPE培养及后续体内移植的理想界面。这种新型装置表现出比常规注射供体细胞更好的生物利用度,可能是治疗AMD的替代方案。
文章内容(英文)
Background:\nAge-related macular degeneration (AMD) is one of the leading causes of vision loss. Once the retinal pigment epithelium (RPE) layers are destroyed, the poor visual acuity and recognition are generally irreversible. Cell therapy that possesses enormous potential in regenerative medicine may provide an alternative treatment for several incurable diseases such as AMD. In this study, we developed an innovative polydimethylsiloxane (PDMS)-based biomimetic scaffolds with cylinder micropillars for the cultivation of induced pluripotent stem cell–derived RPEs (iPSC-RPEs). RPEs were cultured on the PDMS-based biomimetic scaffolds and validated the cells gene expression.\nMethods:\nThe biomimetic PDMS scaffold was fabricated through spin coating and lithography method. It was further modified on surface with biomolecules to improve cell affinity and stability. The iPSC-RPEs were seeded on the scaffold and analyzed with characteristic gene expression.\nResults:\nPDMS biomimetic scaffold was analyzed with Fourier transform infrared spectroscopy and proved its chemical composition. iPSC-RPEs demonstrated confluent cell monolayer on the scaffold and maintained RPE-specific gene expression, which proved the PDMS-based biomimetic scaffold to be supportive for iPSC-RPEs growth.\nConclusion:\nThe PDMS interface allowed regular growth of iPSC-RPEs and the design of cylinder micropillars further provided the bioscaffold high motion resistance may improve the engraftment stability of iPSC-RPEs after transplantation. Taken together, this innovative PDMS-based biomimetic scaffold may serve as an ideal interface for in vitro iPSC-RPE cultivation and subsequent transplantation in vivo. This novel device exhibits better bioavailability than conventional injection of donor cells and may be an alternative option for the treatment of AMD.
来源出处
Journal|[J]Journal of the Chinese Medical AssociationVolume 83, Issue 11. 2020. PP 1029-1033
DOI
https://doi.org/10.1097/JCMA.0000000000000428

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产品颜色:保质期限:36个月

存放环境说明:室温,阴凉处保存

备注说明:

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